miRInform™ Data Delivery System
miRInform™ was designed to help clients understand and manipulate the data generated
by miRNA Expression Profiling. The results of your study are organized and delivered
in an easy-to-access format.
Explore your data by selecting straight from interactive data graphs and tables
to review miRNA database entries on the most differentially expressed miRNA, sortable
tables, and online help files.
Description of Services
- Features of the miRInform System:
- Understand your data within minutes
- Fast, cost-effective analysis for a wide range of experimental
designs
- Normalization optimized for miRNA analysis
- Interactive figures linked directly to
Sanger miRBase
- Publication-quality images included
- Know your signal processing and summarization were performed appropriately
- Available for the
DiscovArray and
Agilent miRNA profiling services
- Workflow for the miRInform System:
- Data summarization (if necessary)
- Normalization by VSN
- Calculation of QC metrics
- Statistical analysis by one-way or multi-way ANOVA
- Generation of reports and graphs
Deliverables
- Raw array data files (see
miRNA Services)
- Bioanalyzer PDF files (for projects including RNA isolation services
from non-FFPE samples)
- Analysis Report Tables (in HTML format and MS Excel format, unless
table dimensions exceed MS Excel capabilities, in which case tab delimited files
will be provided)
- Experimental design
- Array QC Summary
- Control Signals
- Raw Signal and Detection calls
- Statistics (not included if experimental design does not allow statistical
analysis, or in data-only projects)
- Pearson Correlation coefficients
- Probes Detected by Source
- Normalized Array Data
- Comprehensive Annotation to the most recent release of Sanger miRBase
- Figures
- Static figures (in .jpg, .eps and .png format):
- Data enhanced volcano plot(s)
- Data enhanced MA plot(s)
- Hierarchical Clustering, all detected probes
- Pearson Correlation coefficients
- Principal Components Analysis (PCA) Plot(s): A) All detected probes
and B) All probes passing significance testing if experimental design allows statistical
analysis
- Box Plots: Before and after normalization
- Interactive figures (Java™ enabled) - High quality figures are
ready for publication directly onto your desktop or PowerPoint. Spend your valuable
time reviewing the results rather than generating figures from your data.
- Volcano plot(s) with hover-over probe identification and hyperlinks
to Sanger miRBase
- MA plot(s) with hover-over probe identification and hyperlinks to
Sanger miRBase
- Hierarchical Clustering plots with interactive drill-down enhanced
visualization
- PCA Plots with hover-over sample identification
Example Volcano Plot from miRInform:
miRInform contains many data-rich volcano plots, which show p-value and fold change
between two groups for all miRNAs. Color and spot diameter add information about
mean expression level and percent detected, respectively in static plots. Validated
Sanger content is hot linked to the Sanger miRBase.
Threshold and Normalization
For experiments involving hypothesis testing, miRInform offers unprecedented value.
miRInform captures the results from Asuragen’s statistical analysis for a wide range
of study designs.
- Recommended thresholds for p-value and fold-change are
provided, but these can be adjusted for your unique study needs
- Recommended normalization method is global in nature to maximize
precision within a study, but array normalization also can be specified
- Array-based normalization instead if it suits your downstream
analysis
- Optimized for experiments with fewer than 100 samples and 10 experimental
groups
- Our informatics staff can easily accommodate any study design
and size
Confused about these choices? Contact us for a
free consultation to determine what is right for you.
Quality Control
Rigorous procedures enable Asuragen to be the only service provider to offer a Data Quality Guarantee:
If, for any reason, a qualified sample does not pass our process control specifications,
we will re-run the sample at no additional charge.
Read more about our commitment to the
best possible data quality, or read about our contributions to in-process controls and standardization.
Turn-around time
Most projects are completed in one week or less. If you have a critical time requirement,
we can often meet your need for no additional charge.
Read more about our commitment to prompt turn-around times.
Additional Services Available
Catalog Numbers for miRNA Informatics Services
Pathway Analysis:
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CS0004
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Data Analysis, microRNA, Standard Service Premium
|
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CS0020
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Data Analysis, miRInform™ package
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